Journal: The Journal of veterinary medical science

Article Title: Hexachlorophene and cuprizone induce the spongy change of the developing rat brain by different mechanisms: the role of 2', 3'-cyclic nucleotide 3'-phosphodiesterase (CNPase).

doi: 10.1292/jvms.11-0469

Figure Lengend Snippet: Fig. 1. Oligodendroglial apoptosis characterized by fragmented nuclei and condensed eosinophilic cytoplasm in the corpus callosum following CPZ dosing on days 6 (A, arrow), 8 (B, arrows), and 24 (C, arrow). Immunostaining of CNPase in the cerebral cortex in rats treated with HCP (D to F) or CPZ (G to I). Immunostaining of MBP (J to L) in the corpus striatum and caspase-3 (M to O) in the corpus callosum following CPZ treatment. CNPase reactivity is observed in the submembranous region of oligodendroglia in the controls (D), HCP-treated rats on day 5 (E), and CPZ-treated rats on day 24 (I). Strong CNPase immunostaining extends to the swollen cytoplasm of oligodendroglia in HCP-treated rats on day 12 (F) and CPZ-treated rats on days 6 (G) and 8 (H). Corpus striatum nerve fibers stain positively for MBP in the control (J) and recovered animals on day 24 (L). However, immunoreactivity in the corpus striatum decreased on day 8 (K). Caspase-3 expression by some oligodendroglia is observed on days 6 (M, arrows), 8 (N, arrows), and 24 (O, arrow). On day 8, numerous oligodendroglia are strongly positive for caspase-3 (N, arrows).

Article Snippet: Primary antibodies, their working dilutions and suppliers were as follows: mouse monoclonal antibodies against human CNPase (1:400 in dilution, Millipore, Billerica, MA, U.S.A.), human MBP (1:25 in dilution, Serotec Ltd., Oxford, England), PLP (1:200 in dilution, Millipore, Billerica, MA, U.S.A.), and a rabbit polyclonal antibody against activated caspase-3 (1:80 in dilution, Millipore, Billerica, MA, U.S.A.).

Techniques: Immunostaining, Staining, Control, Expressing